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The section was printed before the wildfires began.
Make sure you place the label before the section being labeled, and another label following it so that the computer understands what section is labeled!
Before long the section is packed.
Before reading the section, be sure to skim it.
As already pointed out before, the previous section was devoted to listing convenient conditions on the binary map k in the aim that (3.1) defines a mean.
The isopropanol was evaporated and fresh paraffin was then replenished several times at 60°C before the sections were moved to room temperature (RT).
Three further 10 min washes in PBST were performed before the sections were incubated in freshly prepared 3,3′-diaminobenzidene solution (Vector Laboratories Ltd). until colour development.
Sections were blocked with 5%% goat serum and 0.1 % BSA for 20 min before the sections were incubated with the paralog-specific antibodies (1 400 dilution).
Finally, tissue sections were incubated with DAB (Sigma-Aldrich) until a brown color developed, before the sections were counterstained with hematoxylin.
