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None of the patients were treated with inhaled beta-adrenergic agonists before the sampling procedures.
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All the individuals included in the sample bank were informed by members of the research team about the aims and ethical guidelines of the project and signed an informed consent form before the sampling procedure, conducted by mouth scrap.
In every case, prior informed consent was obtained before the interview and the sampling procedures were undertaken, and participants were given an explanation of the methodology, aims, and expected outcomes of the study, following the ethical guidelines adopted by the American Anthropological Association [ 21].
Subjects residing in the mountain municipalities located in the southern part of the two provinces were excluded from the population databases before carrying out the sampling procedure.
For JA elicitation, plants were treated for 8 days before the start of the sampling procedure by spraying every 2 days with a solution of 100 μM JA (Duchefa, Haarlem, The Netherlands) containing 1.5 mM Tween20 (MP Biomedicals) and adding every 2 days 5 ml of 100 μM JA to the soil.
The sampling procedure is adaptive.
Therefore, the method is easily integrated in the daily routine; AFM could be performed after deparaffination and before the sample staining procedure without diagnostic relay.
There is a linear degradation of accuracy to reproduce bioactive conformers both before and after sampling procedures.
Matuszewski et al. [ 184] described a validation strategy where (i) neat solvents were spiked, or the sample matrix was fortified with analytes (ii) after or (iii) before the sample extraction procedure.
As before the entire down sampling procedure was repeated 100 times.
The experimental design and sampling procedures were approved by the Nanjing Agricultural University Institutional Animal Care and Use Committee before the beginning of this experiment.
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