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given 15 min before the sampling phase impaired novel object recognition evaluated 10 min or 24 h later.
Microinfusion of SKF81297 (0.025 or 0.05 μg/side) into the prelimbic sub-region of the medial prefrontal cortex (mPFC) in this case 10 min before the sampling phase also impaired novel object recognition memory, suggesting that the mPFC is one important site mediating the effects of D1 receptor stimulation on visual recognition memory.
was injected 15 min before the sampling phase of the NOR procedure (see below).
On days 2, 4 and 6, rats underwent a re-acclimatisation of 3 min to the arena before receiving one of three bilateral mPFC infusions 10 min before the sampling phase: saline, 0.05 μg (0.025 μg/side), 0.1 μg (0.05 μg/side) of the selective D1 receptor agonist SKF81297.
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Infusion of a BDNF-blocking antibody into the DG 15 min before the sample phase impaired retention in the s-SLR condition 24 hr later compared to infusion of a control antibody.
Since both encoding and retrieval were affected, the experiment 4 infusion study used the experiment 1 timeline (infusion before the sample phase and a 10 min retention interval) which does not distinguish effects on encoding and retrieval.
The same treatments also reduced novel object recognition memory tested 24 h after the sampling phase and when given 15 min before the choice session.
To test this hypothesis, rats were injected with a BDNF-blocking antibody into the DG before or after the sample phase.
BDNF has been shown to be essential for memory consolidation in several kinds of tasks (Alonso et al., 2002; Lee et al., 2004; Linnarsson et al., 1997; Mizuno et al., 2000), and thus we focused our experiments first on the consolidation as well as the encoding phase of the task by blocking BDNF function in the DG with a BDNF-blocking antibody either before or after the sample phase.
A removable central partition was used during the sample phase but not the test phase of each trial.
The testing phase was performed 24h after the sample phase.
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