Sentence examples for before the sample loading from inspiring English sources
Before the sample loading, the resin was washed twice with ten column volumes of binding buffer (pH 8.0, 50 mM sodium phosphate, 300 mM NaCl, 10 mM imidazole).
After the sample loading, the C18 column was washed for 35 min with solvent A, before applying the LC gradient.
The sample loaded was weighed before and after each cough maneuver.
The sample loaded foam was compressed before measurement.
Purified CtpS protein was incubated at 37°C for 20 min in 50 mM Tris HCl (pH 7.8) and 10 mM MgCl2. 1 mM CTP (Epicentre. Madison, WI) was added immediately before the sample was loaded into the fluorometer.
Before sample loading, the cartridge was conditioned with methanol (1 mL) and water (1 mL).
The Ni2+ column was first balanced with binding buffer before sample loading and then sequentially washed with binding buffer containing 0 mM, 30 mM, 40 mM, and 70 mM imidazole.
Before the samples were loaded on to the SDS/PAGE gels, 40 μg of total protein was mixed with loading buffer and denatured for 5 min at 100°C in a water bath.
Each assay well was first rinsed with 100 μL wash buffer (1× L-WB) before sample loading.
A buffered hydrofluoric acid etch was used to remove the native oxide before the samples were loaded into a high vacuum (4 × 10-6 mbar) thermal evaporator.
