Sentence examples for before the sample injection from inspiring English sources

Mobile phase was injected as the blank solution before the sample injection, and the relative standard deviation (%RSD) values of the peak area and retention time were determined.

When the last fraction of CMC column 1 was being analyzed, the position selection valve was switched to CMC column 2 for equilibration before the sample injection.

Before the sample injection, the programmed Tecan washed with several solutions containing first 1 × SSC, then 0.1 × SSC 0.014% SDS, then 5 × SSC, 0.01% SDS, and 0.2% BSA.

The sample injection volume was 20 μL.

Solvent B was then held at 80% for 5 min to wash the column and then returned to 1% solvent B for equilibration before the next sample injection.

The column was washed for 15 min at 95% mobile phase B and then equilibrated to 100% mobile phase A before the next sample injection.

The syringe cleaning procedure comprised a washing step before and after sample injection, using methanol as washing solution.

The retention of the stationary phase before sample injection was 85%.

Before sample injection, the needle was washed in the flush port with acetonitrile/water (50 50, v/ v) for 5 s.

The gradient was: 0 1 min, isocratic at 50% A, 50% B; 1 12 min, linear gradient to 100% B; 12 15 min, isocratic at 100% B; 15 16 min, linear gradient to 50% A, 50% B; to return to the initial conditions before another sample injection at 20 min.

After peptide elution, the column was flushed with 95% buffer B for 10 minutes and re-equilibrated with 95% buffer A (0.1% (v/v) formic acid) for 12 minutes before next sample injection.