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All rats were carefully examined before the procedure using a slit lamp microscope and the corneas with any defects, new vessels, or cataract were excluded.
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The main objective was to evaluate the patient comfort before after the procedure using a scale of comfort + the secondary objectives were to test the patient and caregiver comfort scores and evaluate in the impact on consumption of analgesic.
Esophageal pull-through manometry was done in all cases before and after the procedure using the Polygraf ID (Medtronic Functional Diagnostics, Denmark).
The interviews were conducted before the ablation procedure using a structured questionnaire guide.
Per analysis day the instrument performance was checked before the measurement procedure using calibration oil as a standard (PRA Coating Technology; Hampton, UK).
Standard commercial practice meant the duck feather and down bedding had been prewashed in hot water, chemically treated and hot air dried by the manufacturer before use, with documentation of the procedures used (Myer House Brand, Hangzhou, China).
After pretreatment and antigen retrieval as described above, the slides were incubated for 60 min at room temperature with the undiluted monoclonal antibody (6C9) before completion of the staining procedure using the Catalyzed Signal Amplification System (Dako Cytomation) in line with the instructions from the manufacturer.
A live dead assay was then incubated for 15min in the dark before microscopic procedure using Zeiss "Axiovert S 100" and filter set #09 (Carl Zeiss AG, Jena, Germany).
Also, nest counts outside the main sinusoid were calculated separately before the fitting procedure used by Gratiot et al. [ 19].
We did measure glucose fluxes before and during the clamp procedure using deuterated glucose infusion.
This study aimed to evaluate quantitatively the time course of changes in ciliary body thickness and AC depth (ACD) before and after the scleral buckling procedure using ultrasound biomicroscopy.
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