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JMV2959 (1, 2, and 3 mg/kg, i.p). was administered 20 min before the operant session, once the rats had maintained a stable level of responding for 5 weeks (∼25 FR3 sessions).
To evaluate the acute effect of JMV2959 on sucrose self-administration, JMV2959 (1, 2, and 3 mg/kg) or vehicle (saline) were administered 20 minutes before the operant session.
Mice were weighed once a week before the operant session.
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A house light was present on the wall opposite the levers and remained on at all times during the operant session.
Conditioned suppression of morphine seeking was measured in the same way during the operant session following fear conditioning.
Mice were exposed to the CS during the operant session preceding fear conditioning in order to measure the baseline suppression caused by the CS alone.
Before the operant box sessions, the mice were weighed to determine their pretrial weight.
The 1st operant session was conducted at the beginning of the dark cycle on the next day.
Mice were allowed to self-administer morphine in an operant session on the first day of each week, and they were given unlimited access to both morphine and water in their home cages on days 2 5 (Fig 2A).
As the effects of coherence and target value diminish during the delay period, the effect of choice continues to grow, reaching its peak immediately before the operant saccade.
All injections were given as an acute i.p. injection (1 ml/kg for rats and 10 ml/kg for mice), 30 minutes before bottles were presented or before the start of the operant self-administration session.
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