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After each enzyme run, the lipase containing PD-MNPs were magnetically isolated and washed twice with hexane and ultrapure water to remove any remaining substrate and product species before the next experiment.
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The injection system was purged with air before running the next experiment to remove the pulverized coal that remained in the pipe of the blast furnace.
For the SILAC experiments, A2780 cells were cultured in "light" (C6 N4-arginine and C6-lysine, SILANTES) and "heavy" (C6 N4-arginine and C6-lysine, SILANTES) conditions for eleven passages before the next experiments.
As explained, for the clarity of the paper, the Materials, Methods and Results are presented for each experiment before going to the next experiment.
Because of the numbers of experiments and the use of three separate cohorts of rats, the results for each experiment are described before progressing to the next experiment.
Darwin transferred the drawing to a sheet of paper before cleaning the glass for the next experiment.
In the next experiment, animals were treated 2 h before LFS with the protein translation inhibitor, anisomycin (240 μg, i.c.v).
The next experiment is "power assist only".
The next experiment refers to the real subset.
In the next experiment, we consider different buffer sizes.
The next experiment addresses these concerns.
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