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However, when the contrast polarity is reversed (from contrast increments to decrements), an unadapted response is observed before the neuron again rapidly adapts to the new polarity stimulus.
This indicates that the dendritic spine density was not altered before the neuron was lost and therefore spine loss did not precede layer III neuron loss.
The three blockers of nitrergic transmission depolarized B31/B32 by a mean of 24.9±8.9 (SD) mV (Fig. 3F) before the neuron began to fire.
Before the neuron's firing is quenched (when F>F*), even though the output firing rate is increasing over some segments of the input range (due to the phase locking under this parameter region, see Appendix S2 for a detailed explanation), its overall trend is decreasing.
At later timepoints 16-244 h), membrane blebbing and extensive neurite degeneration occur shortly before the neuron starts to lose its structural integrity.
Turnover of active signaling molecules would have to be fast relative to the time between different developmental steps, so that the old signal dies down before the neuron is ready to interpret a new signal for a different step.
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I've spent hours staring, with furrowed brow and eraser-tipped pencil, at a clue like "Leather mask put on after conflict about face" -- rearranging the letters in a word, erasing them, then deciding that my first instincts were right -- before the neurons finally fire in the proper sequence and I realize that the answer is "rawhide".
P5 SCG neurons were grown overnight without NGF in medium containing Boc-D-FMK before the neurons were treated with either proNGF or mNGF.
Here we purposefully analyzed only the non-saturating part of the FI curve, most often stopping current steps before the neurons go into block.
We next quantified CNTFRα immunoreactivity in Cre-expressing MNs at 2 weeks post-injection in an effort to 'catch' some of the floxed neurons after their CNTFRα levels are detectably depleted but before the neurons die and are lost from the analysis.
As before, the number of neurons displaying bAPs jumped from less than half at baseline, up to 100% during application of 40% hACSF).
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