Sentence examples for before the isolation of from inspiring English sources

Before the isolation of insulin in the 1920s, most patients died within a short time after onset.

Selected cultures were treated with 1 mM DEANO for 30 min before the isolation of the RNA.

Because at least two azole drugs were brought to market (ketoconazole and miconazole) before the isolation of YJM789 and other clinical strains [6], it is intriguing to observe that a yeast strain with high azole sensitivity could still survive in the pathogenic S. cerevisiae population.

For experiments involving inhibitors for the c-jun NH2-terminal kinase (JNK) or mitogen-activated protein kinase/ERK kinase (MEK), cells was co-cultured with the JNK inhibitor SP600125 (50 µM, A. G. Scientific, Inc., San Diego, CA, USA) or MEK inhibitor PD98059 (50 µM Sigma, St . Louis MO, USA) for 24 hr before the isolation of RNA for RT-PCR assay.

Before the isolation of mitochondria, the seed cover was removed.

MSCs, NPCs and ACs were thawed and expanded 6 days before the isolation of NCs.

Similar concentrations of ampicillin were reported before for the isolation of ampicillin-resistant bacteria [31].

The CcRBCS1 gene was amplified from genomic DNA (10 ng) using the primer pair 18244 (Table 1) and PCR conditions identical to those described before for the isolation of CcRBCS1 cDNA.

– Campylobacter infection was defined as asymptomatic if there were at least five consecutive symptom-free days before and after the isolation of Campylobacter from faeces.

The sera of cases were gathered before (almost 5 days before), during and after the isolation of yeasts from blood culture, defined as day 0 (D0).

Once a match ends, the players shake one another's hands before slipping off into the isolation of endless downtime that is rarely seen by their adoring fans.