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4. Cook the pasta al dente, following timing instructions on the package but checking a minute before the indicated time.
Cook al dente following the timing directions on the package but checking about a minute before the indicated time.
Cook al dente, following the timing instructions on the package but checking the pasta a minute before the indicated time.
Cook al dente, until firm to the bite, following the cooking instructions on the package but checking the pasta a minute or two before the indicated time.
Two hours before the indicated time points, cells were washed 2 times in 0.01 M PBS and the media was replaced with 400 µl of fresh media.
Questionnaires were mailed approximately 1 week before the indicated time.
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Absorbance readings were taken at 0, 248 48, and 72 hours by adding WST-1 (Roche Diagnostics Division de Hoffman La Roche Limitée, Laval-des-Rapides, QC, Canada) as a cell proliferation reagent to each well (10% WST-1 in Dulbecco's Modified Eagle's Medium) followed by incubation at 37°C for 2 hours before reading at the indicated time points.
Mice received an injection of glucose (2 g/kg body wt), and blood glucose was assayed immediately before and at the indicated time after glucose administration.
Mice received an injection of human regular insulin (2 units/kg body wt; Novo Nordisk, Copenhagen, Denmark) into the intraperitoneal space, and blood glucose was assayed immediately before and at the indicated time after injection.
Cells were seeded 24 h before treatment for the indicated time periods with TRAIL.
High magnification images of adherens junction before and at the indicated time points after photobleaching.
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