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Cells were returned to the incubator for a brief period to recover before the imaging session (15 45 minutes).
To image angiogenesis we employed the constitutively emitting AngioSense 750 probe and injected the mice just before the imaging session.
Ten minutes before the imaging session the mice were injected with 100 µl of the AngioSense-750.
Before the imaging session, the cuticula, the glands, and the trachea above the AL were removed.
The animal's eyes were covered with cotton pads and NRR during the surgery until immediately before the imaging session.
Naïve mice never encountered the odorants used for behavior testing before the imaging session whereas the passively exposed group of mice received the same amount of trials and stimulus exposure than the trained group.
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After the imaging session (18.7 ± 12.6 days), each patient underwent a radical prostatectomy and the excised prostate was sliced at 4 5 mm intervals before undergoing tissue processing.
The typical imaging session has duration of 60 90 min. throughout the imaging session the mouse is alive and anaesthetized.
Animals received continuous inhaled isoflurane (1.5 2%) for the duration of the imaging session (10 15 minutes).
During the remainder of the imaging session anaesthesia was maintained with 1.5 2.0% isofluran.
All subjects provided written informed consent prior to the imaging session.
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before the learning session
before the research session
before the test session
before the founding session
before the imaging procedure
before the imaging scan
before the simulation session
before the counselling session
before the operant session
before the training session
before the imaging component
before the imaging acquisition
before the interpretation session
before the dialysis session
before the testing session
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