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All mice were sedated with isoflurane before the imaging.

Cells were pre-treated with 0.05 µM CHIR99021 (Cayman Chemical, Ann Arbor, MI, USA) or DMSO control 24 h before imaging and during the imaging analysis.

Ideally, the surgeon would apply the colored inks subsequent to imaging, but before removal of tissue from the imaging platform.

Positive oral contrast agent may also be administered as part of the imaging preparation 60 min before imaging if lower abdominal and pelvic delineation of bowel loops from adjacent structures is required.

Before MRI, both parents are informed about the imaging procedure and possible implications of imaging findings.

All subjects took potassium iodide orally (three doses of 40 mg on the day before imaging and 80 mg just before imaging) to block thyroid uptake of free radioactive iodide.

After ingestion of potassium iodide (three doses of 40 mg on the day before imaging and 80 mg just before imaging) to block thyroid uptake of free radioactive iodide, the selective radiolabelled D2R antagonist [I]IBZM was infused using the sustained equilibrium/constant infusion technique [ 14, 19].

Excess sample was flushed out of the chamber before imaging.

are used to test the system before imaging live tissues.

siRNA transfection and thymidine synchrony was performed as described in Fig. 1 except that upon release from the second thymidine arrest or before imaging (if imaging asynchronous cells) the standard medium containing the thymidine was exchanged for DMEM without phenol red, supplemented with 10% FBS, penicillin/streptomycin and 200 mM Trolox (Calbiochem).

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