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Finally, DNA was stored at −80 °C before the further analysis.
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Samples for histological examination were removed before freezing and analysed independent of the further analysis of RNA.
A total of six landraces (Alaku-samba, Batapolawee, Heendikwee-1, Kahatawee, Madael and Morogallawee) which produced unusually long branches in the dendrogram were removed from the list before further analysis to determine the number of populations within the Sri Lankan accessions.
Therefore, the samples of patient 11 were removed from the dataset before further analysis.
The EST sequences representing the tPA gene (98 sequences) were removed from the dataset before further analysis.
A big challenge is how to correct for the bias before further analysis.
Contigs with successful hits (E-value cut-off of 1×10-25) weremovedved from the assembly before further analysis.
The activated B cells were positively selected with biotinylated anti-CD19 antibody (BD Biosciences Pharmingen) conjugated to streptavidin beads (CELLection Dynabeads; Dynal) and released from the beads before further analysis.
Contigs with successful hits (E-value cut-off of 1e−6 and >95% identity) were removed from the assembly before further analysis.
The detection (Present/Absent) call generated by the Affymetrix microarray suite version 5 software (MAS 5.0) was used to remove probe sets that were not reliable detected in any of the microarrays before further analysis.
Therefore, we performed a batch correction for both cell types using the following ANOVA model before further analysis of the data: y i = μ + B i + e i where y i is the expression level of the ith microarray, μ is the overall mean, B i is the batch to which the ith individual belongs and e i is the residual error.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com