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The movie contains 20 TIRF images of T24 cells expressing GPI-hFR-mGFP, which were recorded at 1 min intervals; the overview shows an area of ~ 400 × 400 μm. 100 μM KLK was added immediately before the first image.
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It is likely to be several days before the first images are processed and released.
Integrated fluorescence intensity of the converted fluorophore (red) was measured in the bud for every time point and after subtraction of the background normalized to 10 for the first image (before conversion).
To analyze intracellular VAMP-2 distribution, two frames from the experiment were selected for analysis: the first image from directly before the stimulation and the second image from a point around 1 3 minutes after the stimulation where protein condensation (appearing as bright spots) appeared the greatest.
The first image shows ANO6-EGFP fluorescence obtained before establishing whole-cell recording.
The first image is especially haunting.
The first image feels right.
Hold the first image as a snapshot.
Delete the first image you created.
The second image will appear behind the first image.
A Nasa spacecraft that dived through a geyser plume on one of Saturn's moons, closer to the surface than ever before, has delivered the first images and data from its "taste" of an underground ocean.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com