Sentence examples for before the expression was from inspiring English sources

An outsider musician long before the expression was coined, Fischer made up nursery rhyme-like ditties such as "Merry Go Round", "Monkeys Versus Donkeys" and "Which Way Did the Freaks Go?", which he mostly sang a cappella in a raspy voice that often wavered from the intended melody and tempo.

The results indicated that αvβ3 was selectively expressed on CD16- monocytes in the presence of M-CSF + RANKL, and the expression was revealed before the cells differentiate into typical multinucleated osteoclasts.

(The expression was Fuller's before it was Dickinson's; the poet is said to have loved Fuller's work).

The second group was composed of 38 miRNA families that are lowly expressed before the 5.3 hpf, while the expressions are dramatically increased in later stages.

As before, the decline in expression was not associated with an increase in serum transaminase levels or with the development of inhibitory Abs against FVII, as assessed by ELISA and a modified Bethesda assay.

Compared to pancreatic cancer tissues before chemotherapy, the SIRT1 expression was clearly upregulated in pancreatic cancer after chemotherapy in mRNA (12.14 ± 6.68 vs 2.96 ± 2.06, P < 0.05) and protein levels (0.88 ± 0.41 vs 0.22 ± 0.10, P < 0.05) (Fig. 9b,c).

This suggests that either the replum identity is already established prior to or around stage 10 before the FUL GFP expression is basal-apically up-regulated in the replum, or that for the conversion of replum to valve cells FUL protein needs to be present in all cell layers of the replum.

Before differential expression was assessed, a nonspecific filter was applied to reduce the number of probe sets in each comparison.

Transfected cells were incubated for 48 hrs. at 37°C with 5% CO2 before luciferase expression was measured.

Keratinocytes were further cultured for 4 days before shRNA expression was induced by treatment with doxycycline.

The level of gene expression within each sample was adjusted to an internal control (human ribosomal 18S) before expression was calculated as a percentage of the level of gene expression by the control sample.