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After this challenge, preparations were left undisturbed for 30 45 min to stabilize before the experimental protocol was initiated.
Since skull drilling induces vasodilation, we allowed the animal to recover for 1 h before the experimental protocol.
Afterwards, the sheep were turned into prone position and were allowed to recover for a period of 30 min before the experimental protocol was started.
After surgery, the animals were injected with a dose of 100,000 U of benzyl-penicillin intramuscular as antimicrobial prophylaxis, and were allowed a postoperative recovery for 1 week before the experimental protocol.
We allowed 1 week for acclimation before the experimental protocol began.
Of note is that these lean dogs had normal insulin sensitivity before the experimental protocol to induce insulin resistance; hence, the results represented an early development of insulin resistance.
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Female NOD mice (4 weeks old) were purchased from the Laboratory Animal Resource Center of the Peking University Medical Science Center and were acclimatized to the center for 1 week before starting the experimental protocol.
Therefore, overall the study duration was 7 h or 420 min. Each subject voided to empty his/her bladder before starting the experimental protocol.
The day before starting the experimental protocol (D0), the groups consisted of Group 1: 6 calves (3 males and 3 females) that were 60 ± 0 days old and weighing 77 ± 5 kg.
Animals were acclimatized to the laboratory, exposure chamber, and all aspects of the experimental protocol before beginning experimentation.
Rats were purchased from the Institute for Experimental Research and Application (Istanbul Medical Faculty), and were cared for before and during all stages of the experimental protocol in compliance with the applicable institutional guidelines and regulations of the Institute for Experimental Medicine Research and Application.
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