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Temperature calibration was performed before the experiment using a thermocouple.
Quality control measurements on the cells were recorded before the experiment using a bench cell analyzer sorter (Guava technology).
Endophyte infection status of nine plant individuals per field plot was verified before the experiment using immunoplot assay to detect monoclonal antibodies specific to Neotyphodium (Phytoscreen Immunoplot Kit #ENDO7973, Agrinostics, Watkinsville, Georgia, USA).
In brief, the subject was fit-tested before the experiment using the Portacount method (TSI, Inc., St . Paul MN).
Fundamental experiments to determine the range of each of these factors were performed before the experiment using a statistical model.
The absorption spectra of the two chromophores were calibrated before the experiment using a spectrophotometer (USB2000, Ocean Optics, FL) (Fig. 5(a) ).
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TDR sensors were calibrated before starting the experiment using soil samples of the location under study with known water content.
For experiments using palonosetron (0.01 nM–3.0 μM) the antagonist was incubated for 45 min with the cells before starting the experiment using an EC50 concentration of 5-HT (unless otherwise stated).
Loss of sensation during INB and SB was tested before the second half of the experiment using skin touch and passive movements of the limbs as described previously.
The fluidic layer was flushed by the phosphate buffer supplemented with Tween 20 at 0.02% before the experiments, using a syringe pump (Fusion 400, Chemyx, Stafford, TX).
However, teachers should be careful to explain the meanings of each term before they perform the experiments using our protocol.
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