Sentence examples for before the experiment cells from inspiring English sources

Forty eight hrs before the experiment, cells were split into MEM supplemented with FBS and antibiotics but without G418.

One day before the experiment, cells were suspended in 200  μl of medium in a 96-well plate at a concentration of 5000 cells per well.

One day before the experiment, cells were washed with PBS (1×), medium changed to HepatoZYME-SFM (Gibco) and cultured for further 24 hrs.

Before the experiment cells were seeded in RPMI 1640 media without phenol red, and differentiation was activated by overnight incubation with PMA (50 pmol/10 cells).

Before the experiment, cells were incubated for 1 h in KRH buffer (50 mM HEPES, 136 mM NaCl, 23.5 mM KCl, 1.25 mM MgSO4, 1.25 mM CaCl2 and 0.1% BSA).

One hour before the experiment, cells were incubated in a DMEM medium without serum supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10 mM HEPES, with or without 25 mM glucose.

Between 16 and 20 h before starting the experiment, cells were transfected with GFP fusion constructs using polyethylenimine according to manufacturers protocol (JetPei, Qbiogene).

Before the experiments, cells were incubated for 45 min with FURA-2 (Molecular Probes) in complete DMEM medium, followed by three washes with calcium saline buffer (138 mM NaCl, 6 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 5.5 mM glucose, 0.1% bovine serum albumin, pH 7.4).

Immediately before the experiments, cells were washed twice in glucose-free sperm cell medium.

Immediately before the experiments, cells were incubated in DMEM alone for 1 hr.

The day before the experiments, cells were seeded in six-well plates at concentration of 2.5 × 10 cells per ml.