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pretreatment with anti-IL6 antibody before the exercise protocol blocked these effects in the hypothalamus of exercised DIO rats (Figures 7e and f).
In the placebo treatment, a capsule filled with maltodextrin was administered before the exercise protocol and at the 90 minute time point.
A 300 mg dose of a NDRI (bupropion) was administered before the exercise protocol, and a placebo pill was given 90 minutes into exercise.
Caffeine was administered in two doses – a 2.5 mg · kg−1 body mass dose 1 hour before the exercise protocol, and a 5 mg · kg−1 body mass at 90 minutes into exercise.
pretreatment with anti-IL6 antibody before the exercise protocol, reversed the suppressive effects of leptin on AMPK/ACC pathway in the hypothalamus of exercised DIO rats (Figures 7c and d).
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The treatment started one day before the beginning of the exercise protocol, and continued until the day of sacrifice.
The blood pressure (BP) was measured before, during and after the exercise protocol.
In all experimental trials, participants completed a battery of visual tasks (see below, visual performance measures) before exercise (pre-exercise), and immediately after the exercise protocol (post-exercise).
The sample size of this protocol are reduced because we consider that, before implementing the program in the general community, for public health, the exercise protocol should be validated by a very controlled process.
All tests and measures were collected in a research laboratory setting before exercise and at 24, 48, 96 hours and one week after the exercise protocol was administered.
After the exercise protocol, participants completed the visual task battery.
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