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Before the coupling reaction, we measured the total concentration of HAI-178 antibody.
During the time after the room change and before the coupling trigger, there will be echo blips which would be suppressed by the postfilter through the residual echo.
The resin was then washed with dichloromethane (DCM) and DMF and allowed to swell in DCM for 15 min before the coupling reaction.
In particular : A careful dehydration of the starting PEG and of their oligonucleotide-bound intermediates must always be achieved, especially before the coupling steps, owing to the hygroscopicity of the polymer.
For the disulfide bond, cyclization of LyP-1 was performed on resin with iodine (10 equiv) in DMF for 4 h before the coupling of the hydrophilic linker (N-Fmoc-amido-dPEG4 acid).
A careful dehydration of the starting PEG and of their oligonucleotide-bound intermediates must always be achieved, especially before the coupling steps, owing to the hygroscopicity of the polymer.
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