Sentence examples for before the blocking from inspiring English sources

On CrunchGear.com, one user said he had been able to connect briefly on Skype — "enough to say hello, meet me and goodbye" before the blocking system sniffed out the connection and shut it down.

Tissue sections used for caspase-3 or OMP detection were pretreated before the blocking solution with 3% hydrogen peroxide in methanol to destroy endogenous peroxidase.

For MyoD, myogenin, or myosin heavy chain immunolocalization, PFA-fixed specimens were treated with 0.3% Triton X-100 (FisherBiotech, Fair Lawn, NJ, USA) in PBS before the blocking step.

For detecting the mitochondrial localized proteins, cells were permeablized with 0.25% Triton X-100 in PBS for 10 min at room temperature and washed three times with PBS before the blocking step.

For labelling using the 4G8 (1:500; Covance) antibody, slides were treated with 3%H2O2in50%50% methanol, incubated in 90% formic acid and then washed several times in dH2O before the blocking step, as described previously [ 28].

Whenever the A4 antibody was used, antigen retrieval was performed before the blocking step by incubating the sections in preheated sodium citrate buffer (10 mM, pH 8.5) at 80°C for 30 min.

Briefly, slices were incubated in H2O2 3% for 20 min before adding the blocking mix.

However, in 2 1 AV block, there is one PR interval before the blocked P wave making impossible to distinguish between type I and type II block [2, 5].

The following morning, slides were rinsed in ddH2O 3X before proceeding to the blocking step described above.

Just a day before, the police blocked a group of Jews from marching into the neighborhood.

The strategy has worked before; the Justice Department blocked mergers from AT&T and H&R Block in 2011.