Sentence examples for before the assay using from inspiring English sources

They were counted before the assay using a Burker hemocytometer.

The BD BioCoat Matrigel™ invasion chambers with polyethylene terephthalate-filters coated with matrigel basement membrane matrix (6 wells, 8 μm pore size; BD Biosciences, Franklin Lake, NJ) were re-hydrated just before the assay using FBS-free DMEM according to the manufacturer's instructions.

Differentiation was confirmed before the assays using a monoclonal antibody to CD11b (kindly supplied by Dr. C. Bernabeu, CIB-CSIC) which is a useful marker of granulocytic differentiation [29].

Short hairpin RNA transfections were done 48 h before performing the assays, using the pSicoR vector, carrying a green fluorescent protein expression cassette and containing the short hairpin RNA sequence of choice.

The amount of DNA used in each assay was measured before assay using a Nanophotometer Pearl (Implen GmbH, Munich, Germany) with an AE-labeled telomere probe (Fujirebio Inc., Tokyo, Japan).

The hemicellulose fractions from all biomasses were also diluted 40 times before the immunolabeling assays using LM11.

The conductivity of the EP medium in which all batches of embryos were suspended during OET assay was measured (immediately before assay) using a hand-held conductivity meter (model B-173, Horiba, Japan).

The Amplicor HIV-1 Monitor assay (RT-PCR) [31] yields, on average, lower concentrations than the NASBA HIV-1 RNA QT assay, the only assay used in samples taken before 1996.

Compared to levels of estradiol measured by RIA where extraction via celite chromatography was applied before the assay was used [ 9], levels of total and unconjugated estradiol (LC-MS/MS) were well correlated.

Five micromolars of tetramethylrhodamine ethyl ester (TMRE; Life Technologies) added immediately before the assay were used for ΔΨm monitoring on an RF 5301 PC spectrofluorometer (Shimadzu, Tokyo, Japan) in a stirred 2 ml cuvette with excitation at 561 nm (3 nm slit) and emission at 579 nm (5 nm slit).

Neutralizing antibody titers were determined by standard PRNT assay using LLCMK2 cells as before [ 42, 43].