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Immunoprecipitates were washed twice with RIPA buffer before the addition of 2× Laemmli buffer.
And that was before the addition of that day's catch.
Inhibitors were added one hour before the addition of Hcy.
A control PCL sample was analyzed before the addition of the apatitic component.
Cells were incubated on ice for 10 min before the addition of DiD-HCV (MOI = 1).
Figure 4 Cryo FEGSEM photomicrograph of preformed silver nanoring before the addition of NaBH4.
Before the addition of the enzyme, one sample was measured with the above conditions.
AgNO3 (200 mg) was dissolved in water before the addition of NaOH and formation of precipitates.
The resulting solid was dissolved in methanol, before the addition of aqueous NaClO4.
Scanning electron microscopy shows preformed nanorings before the addition of the reducer (Fig. 4).
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Cells were pretreated with HSME for 2 h before the addition of 100 μM H2O2.
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