Sentence examples for before the absorbance of from inspiring English sources

Then 12.5 μl of Folin-Ciocalteu phenol reagent (50%) was added and allowed to stand for 30 minutes at room temperature before the absorbance of the reaction mixture was read at 750 nm using the UV-VIS spectrophotometer (Analytic Jena Specord 205).

At 30 min time intervals, 100 µl samples were taken and centrifuged for 5 min at 16000×g before the absorbance of the supernatant was measured at a wavelength of 405 nm in an ELISA-reader (Tecan).

The signal was allowed to develop for a minimum of 30 min before the absorbance of each well was read at 650 nm.

The cells were incubated for a further 2 4 h at 37°C before the absorbance of the wells was measured at 492 nm.

The samples were centrifuged at 2,000 g for 5 min before the absorbance of the supernatant (S) was measured at 405 nm by a spectrophotometric microtiter plate reader (Thermomax Molecular Devices, NJ, USA).

Reporters captured on the plate were then detected by adding 100 μL of 0.2 μg/mL neutravidin-HRP (Pierce), developed with 50 μL TMB solution (Thermo Scientific) for 5 15 min, and quenched with 50 μL of HCl before the absorbance of the wells was determined by microplate analysis (SpectraMax Plus, Molecular Devices) at 450 nm.

Before measuring the absorbance of the solution with the spectrophotometer, any bubbles in the samples are removed with a gentle stream of nitrogen.

Before reading the absorbance of each enzyme reaction, the absorbance of the blank was set to 0, in order to omit the background rates caused by random hydrolysis of the p-NP-ester substrates during incubation.

The reaction was initiated by the addition of 120  μL of 5 mM ferrozine into the mixture, which was then left at room temperature for 10 min before determining the absorbance of the mixture at 562 nm.

The absorbance of the samples before and after laser conditioning was measured by surface thermal lensing (STL) technology and the defects density was detected under Nomarski microscope.

{text{Dye}};{text{Uptake}}; = frac{{A_{text{b}} - A_{text{a}} }}{{A_{text{b}} }} times 100 (1)where A b is the absorbance of the dye bath before dyeing and A a is the absorbance of dye bath after dyeing.