Sentence examples for before the absorbance at from inspiring English sources

After 0, 248 48, and 72 h, medium was replaced with fresh medium containing 1/10 volume of cell proliferation reagent WST-1 (Roche Diagnostics) and incubated for 2 h at 37°C before the absorbance at 450 nm was quantified in a spectrophotometer.

The mixture was boiled for 15 minutes and cooled on ice before the absorbance at 464 nm was measured.

The mix was incubated in the dark at 37°C for 4 h before the absorbance at 490 nm was evaluated in an ELISA reader.

Cell-free supernatants of S. oneidensis cultures were mixed 1 1 with the CAS assay solution and equilibrated at room temperature for 2 h before the absorbance at 630 nm was measured.

The samples were incubated for 2 h at room temperature, after which the samples were filtered (0.2 μm pore size) before the absorbance at 760 nm was measured using a spectrophotometer UV-18000, Shimadzu, Kyoto, Japan).

A mixture of DMSO (150 μl) and glycine (25 μl) was added to each well and mixed to ensure cell lysis and dissolving of the formasan crystals, before the absorbance at 540 nm was measured.

The mixture was allowed to stand for 2 min before measurement the absorbance at 340 nm.

The solution was incubated for 5 10 min at 25°C and mixed with 0.2 ml of 7%Na2CO33 solution and incubated for two h 25°C before taking the absorbance at 725 nm.

An aliquot of 8.5 μl of extract was added to 275 μl of diluted FRAP reagent and the samples were incubated at 37°C for 30 minutes before measuring the absorbance at 595 nm.

150 μL of each sample were transferred to a new 96-well microplate before measuring the absorbance at 540 nm using an ELISA micro-plate reader (Multiskan™ GO Microplate Spectrophotometer, Thermo Scientific, Loughborough, UK).

The mixtures were then incubated at 100°C for 12 min before measuring the absorbance at 466 nm as described by Prescott and Jones [14].