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Plasma samples were heat-treated at 56°C for 30 minutes and stored at −20°C for three weeks before testing using a standard haemagglutination test[33].
The pneumotach was calibrated before testing using a 3 L syringe.
Mice were extensively habituated to the maze and to the milk reward before testing using a pair of neutral colored goal arms.
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Mice were fasted for 5 hours before baseline tail vein blood glucose testing using a portable glucometer (Accuchek Aviva; Roche).
To identify the ubiquitination acceptor site within CLS1, a candidate-mapping approach was used where CLS1 lysine mutants were constructed and synthesized in vitro before testing using ubiquitination assays.
The column was first characterised in terms of mixing and gas dispersion, before being flotation tested using a quartz-amine system.
This means the effect on knowledge was tested using a before-and-after study design, without a control group.
The difference of means before and after treatment was tested using a t-test for paired samples.
Differences within groups before and after treatment were tested using a paired Student's t test (two-tailed).
Before injection, the particles were tested using a quantitative limulus amebocyte lysate assay to assure that the endotoxin level was lower than 0.25 EU/mL.
The difference of means before and after treatment was tested using a t-test for paired samples, and the relationship between different parameters was assessed through Spearman's correlation coefficients.
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