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After transfection, iPSCs were seeded and cultured in presence of 10 μM ROCK-Inhibitor for one day before switching to normal culture conditions.
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RPE cells were grown until 70 80% confluent, and then the serum-free DMEM/F-12 was added to the cells for 24 h before switching to high or normal glucose treatment (30 mM or 5 mM D-glucose + 25 L-glucose, resp).
The optional eight-speed autransmissionsmission, a feature never before available on a Z06, executes creamy-smooth gear changes when the car's mode selector is switched to Normal.
At the end of "Waltz with Bashir," we suddenly switch to normal film: genuine news footage from the refugee camps.
We thus switched to normal HEPES solution for later studies.
Chigga!" before switching to "Brian!
He grew cotton for a decade before switching to tobacco.
Before switching to Nascar, she raced in the IndyCar series.
Mr. Citron worked in photography before switching to fashion.
"Merci, cher Philippe," Mr. Holbrooke said, before switching to English.
She began as a heptathlete before switching to sprinting.
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CEO of Professional Science Editing for Scientists @ prosciediting.com