Sentence examples for before supplementing with from inspiring English sources

To make sure you don't overdo (or under-do) the amount, it's important to check with your doctor before supplementing with Vit A and D (or before beginning a supplementation regimen).[17].[17]

Strains AM5708 (BRN1-6HA ), AM10859 (BRN1-6HA pGAL-SGO1 ) were pre-cultured in YEP + R + Ade medium before supplementing with nocodazole and galactose (2 hr) for 3 hr and then harvesting for anti-HA ChIP.

Assay buffer with 100 ml of cell lysate was preincubated for 30 min at 32 °C before supplementing with 1  μM Ac-LEDH-AFC (caspase-9 substrate) with or without 2  μM Ac-LEDH-CHO (caspase-9 inhibitor) and incubated at 32 °C for 24 h.

Seek medical advice before supplementing with creatine.

Always work with a doctor before supplementing with an omega 6 or omega 3 fatty acid if you have disorders or illnesses.

To confirm that downregulation of PDK1 is caused by LPS, cultured podocytes were treated with GIT27 for 2 h before supplementing the media with sera from normoalbuminuric T1D patients with high LPS activity.

However, even though these medications are usually not as "serious" or drastic as the diabetes medications described above, it's usually a good idea to consult with your doctor before supplementing your treatment plan with one of these medicines just in case.

For preparation of GST-CD40 fusion protein extract, E.coli BL21 cells (Invitrogen) were transformed with pGEX-5X-1/CD40 pGEX-5X-1/CD40 pGEX-5X-1/CD40 OD600 ∼0.4 before supplementing the baconstructslture with isopropyl- β- d-thiogandctopyranoside (IPTgrown a final concentration of 0.1 mM for 5 h.

As highlighted by Darveau and coworkers [ 12], before supplementing critically ill patients with iron we need additional studies to investigate and better define the role played by iron, including the place of primordial regulators of iron metabolism such as hepcidin and transferrin receptor [ 19, 20], in the development of anaemia in this population.

Whole roots and the three youngest trifoliates from each plant were harvested and frozen in liquid nitrogen immediately before supplementing the nutrient solution with 1 mM H3BO3 (+B) and then at 3, 6, 12, 24, and 48 hr after B treatment.

Consider supplementing with whey proteins.