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While these collections are of immense value, they do not serve directly as ORF resources, but rather as collections of potential ORFs that must first be subcloned without UTRs before subsequent analysis of the encoded proteins can be performed [12].
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When cells reached 90 100% confluence, they were serum-deprived for 2 72 h in the presence of ascorbic acid (50 µg/ml) before subsequent analysis.
The acellular portion of synovial fluid was stored at -70°C before subsequent analysis.
Sections were deparaffinized in xylene and rehydrated before subsequent analysis.
The entered data were cleaned and edited before subsequent analysis.
Experimental artifacts, both, known systematic errors and confounders of unknown origin, can also contribute to this problem and require adequate preprocessing before subsequent analysis.
To allow comparisons between the assemblies of different assembly programs, singletons and contigs shorter than 100 bp were discarded before subsequent analysis.
The total supernatant was evaporated under nitrogen and then stored in a vial before subsequent analysis.
The pretreated materials were stored at 4°C before subsequent analysis and experiments.
Sorted cells were washed three times with PBS before subsequent analysis or culture in medium.
Hence, the original data versus z was converted to data versus zf before subsequent analysis.
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