GFP plasmid was incubated with lipofectamin for 10-15 minutes before subjection to the cells.
One of them, his publicity chief, North Carolina publisher Josephus Daniels, who became his Secretary of the Navy, spoke for all when he wrote, just before the election, that "the subjection of the negro politically, and the separation of the negro socially, are paramount to all other considerations in the South short of the preservation of the Republic itself".
His greatest success lay in the subjection of the Thuringians, achieved with the help of Chlotar about 531.
A party had been formed in the Carolingian Empire to combat the subjection of the church to the state.
Yet this ancient liberty came at a price that we would find prohibitively high — "the complete subjection of the individual to the authority of the group".
A further series of campaigns led, by 960, to the subjection of the Slavs between the middle Elbe and the middle Oder.
That Bible passage, also known as the "Curse of Canaan," was originally understood to justify the subjection of the Canaanites to the Israelites.
The Civics Badge of 1916 required reading John Stuart Mill's "On the Subjection of Women" and "The Women Voters Manual" "four years before women had the right to vote," said Mary Logan Rothschild, a professor of women's studies and history at Arizona State University and the author of a forthcoming book on the Girl Scouts.
A table of contents follows the memo, and it contains a catalogue of chapters, page numbers, sections, and subjections of the audit.
It is not a neoconservative position to rejoice in the subjection of tyrants to the rule of law.
