Sentence examples for before subjecting the samples from inspiring English sources

To visualise the organisation of the respiratory chain complexes in neutrophils and the control cells, isolated mitochondria from these cells were solubilised with digitonin before subjecting the samples to blue native BN -PAGE.

Ritala et al[ 7] conducted the heat treatment for 6 min before subjecting the samples to 150 μg/mL pepsin digestion at 10°C for 30 min under an acidic condition.

We treated HeLa cells either with 5-azacytidine or with DMSO or 5-aza-2′-deoxycytidine as negative controls for 18 h and labeled proteomic lysates with dimethyl light or intermediate isotopes before subjecting the samples to mass spectrometry (Fig 7A).

The term native TiO2 refers to a thin (10 to 30 nm) TiO2 layer formed due to exposure of the sputtered film to atmosphere and is present before subjecting the sample to anodization.

Before chromatography, they subjected the samples to a fractional crystallization, following several steps: the oil is dissolved and shaken in a mixture methanol-acetone (7 : 3, v/v) and then kept for several hours at −22°C.

Before subjecting a sample to a cooling and heating cycle to form and revert the α′ phase, it was first homogenized for 8 h at 375 °C to remove any microstructural memory of prior transformations.

After washing the beads as before, the samples were subjected to an on-beads tryptic digest and processed for LC/MS/MS analysis.

Before use the samples were subject to the standard cleaning protocol established by Rabung et al. [42]: samples were soaked in acetone overnight, subsequently washed with ethanol, then soaked in ethanol (2 hours), washed with Milli-Q water and finally soaked in Milli-Q water (1 hour).

We measured the duration and slope (rate of force decline) of this linear phase, both before and after subjecting our sample of muscles to a fatiguing stimulation protocol (see Methods for fatigue protocol).

Then, the samples were homogenously mixed before subjected for cell cycle analysis in a flow cytometry machine installed with EXPO32 software (Beckman Coulter Ltd ,UK).

The samples were kept on ice before subjected to flow cytometric analysis.