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On one hand, for urea detection, the urease powder was mixed with the phosphate buffer in a concentration of 1.5 μg/mL, and the urease was dripped on the open window of EIS before storing the sample at 4°C (in the fridge) overnight.
The solution was removed before storing the sample at −80 °C.
Subsequently, the sample was filtered over a 0.22 µm Spin-X filter (Corning) and protein level was determined using a Pierce BCA Protein Assay Kit Pierce Biotechnologyy, Rockford, USA) before storing the sample at –80°C.
Similar(57)
The protein digestion was terminated by adding 50 100 µL of 1% (v/v) trifluoroacetic acid (TFA) and incubating the mixture 2 4 h at room temperature before storing the samples at 4°C until further analysis.
Adipose tissues were removed and stored in RNAlater® solution (Applied Biosystems, USA) and refrigerated at 4°C overnight before storing the samples at −80°C for RNA extraction.
Seawater was removed, and ethanol added before storing samples at -20°C.
Before storing samples at –80°C, urine was aliquoted and specific gravity (SG) was measured using a handheld refractometer calibrated with deionized water before each use (National Instrument Company Inc, Baltimore, MD).
We put observers in the main military garrisons where the boxes of paper receipts were stored, before the sample was drawn, to avoid any tampering with the chosen boxes.
Store the sample correctly.
Drain the liquid before storing.
Check the lids before storing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com