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Then RNase was added to remove RNA before storage of the samples at −20 °C.
However, it should be added immediately before insemination rather than before storage of the sperm dose, to benefit from the transient increase in sperm progressive motility and avoid increased chromatin damage.
Informed written consent of each patient was obtained before storage of SF.
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We therefore evaluated the effect of stage of maturity (mature flowering at 12-week phonological stage) and duration of curing before storage for periods of 2, 12, 24, and 36 weeks on the quality of hay.
(NOTE: Slides should be labeled at the frosted end with pencil before storage to avoid confusion of the side with/without gel) (Bajpayee et al. 2005; Shukla et al. 2014).
The WERF EPHect SOPs recommend using precooled transport/fixation media (where applicable) and keeping samples on ice (4°C) at all times before storage to allow utility of the samples in assays that are both sensitive and insensitive to room temperature conditions.
Through the re-testing of these specimens for HIV, the ICRL detected in one particular site a problem of mislabelling of specimen aliquots before storage, and of clerical errors on the shipment list.
Our diagnostic yield is lower than in previous studies [ 24], this may be due to lack of neutralization before storage and delayed processing of samples.
Fluorescent C.albicans was prepared by adding C.albicans to fluoroscein isothiocyanate (FITC, Sigma) dissolved in sodium carbonate buffer (pH 9.5) at room temperature for 3 h and washed by centrifugation three times in sodium carbonate buffer before storage in aliquots of water at 4°C. Cell lysis and DNA extraction.
feedback at assessment and from laboratory analyses before storage; the modalities of returning general results to participants; and finally, the conditions and modalities of returning individual research results (IRRs) and incidental findings (IFs).
Before storage, all specimens of the EQUIA system were coated with a nanofilled resin (G-Coat, GC, Tokyo, Japan) and light-cured for 10 s using the same ECCO-Light lamp.
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