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Models of the active site before steps 1 and 2 of the splicing reaction predict that exchange of the external G-cofactor and the 3′-terminal G is accomplished by a slight conformational change in P9.0 of the Azoarcus group I intron.
It would seem logical to explore this further before steps are taken to generate FcγRIII-selective mAbs as proposed by Clynes et al, because if patients are unable to mobilise effector cells to sites of metastasis, the strategy will fail.
The study found that the first step in the pathway-to-care was sharing symptoms or signs with family members and friends, and on average, took 271 days before steps toward diagnosis were taken.
The culture preparation is necessary to do these experiments, but addressing these points is important because the data suggest that the cultured neurons do not fire spontaneously (Methods, "only cells with a resting membrane potential more negative than -50 mV were studied"; no indication of holding current to keep cells silent; no spikes evident before steps in current clamp records.
This is the universally accepted major heading on most wikiHow articles (unless you're creating a recipe article where ingredients might come before steps).
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I wondered how many drinks he had before stepping up to the table.
-- before stepping up to the podium.
Comfort, before stepping into the unknown.
He held the position for four years before stepping down.
Before stepping out of the car, the girl hesitates.
– before stepping down into the shin-high waters of Barry Burn.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com