RT-PCR results were normalized against internal controls (GAPDH) before statistical analysis was performed.
Data were validated for completeness before statistical analysis was performed.
As in any data analysis problem, visual inspection of complex data before statistical analysis is vital.
Data were checked for normal distribution before statistical analysis was preformed.
All data were normalized to the housekeeping gene, β-actin (βACT), before statistical analysis was performed.
This was a prespecified secondary investigation of the original CLUE study (reported elsewhere 9), designed before statistical analysis was performed, but after the database was complete and locked.
Samples were assayed on at least three different 96-well reaction plates, often by two different operators, before statistical analysis was performed.
Fluorescence readings from "blank" wells, containing medium and carboxy-H2DCFDA, were subtracted from the raw data before statistical analysis was performed.
The forward primer 799 F was chosen as it is strongly biased against amplifying chloroplast 16S rDNA [ 39]; the much larger mitochondrial 18S fragments were later removed in silico after amplification and restriction, but before statistical analysis was performed.
For all parameters where the variances were significantly different between control and treated mice (namely, tumour volume and tumour weight), the data were log-transformed before statistical analysis was performed.
