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RT-PCR results were normalized against internal controls (GAPDH) before statistical analysis was performed.
Data were validated for completeness before statistical analysis was performed.
Data were checked for normal distribution before statistical analysis was preformed.
All data were normalized to the housekeeping gene, β-actin (βACT), before statistical analysis was performed.
Samples were assayed on at least three different 96-well reaction plates, often by two different operators, before statistical analysis was performed.
This was a prespecified secondary investigation of the original CLUE study (reported elsewhere 9), designed before statistical analysis was performed, but after the database was complete and locked.
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As in any data analysis problem, visual inspection of complex data before statistical analysis is vital.
Standard cut-off values for the different biomarkers, needed to determine categorical scores before statistical analysis, were the same as those published in previous studies [ 26, 27, 30].
The data were checked for normality before the statistical analysis was performed.
All data were anonymized before any statistical analysis was performed and the study was approved by the Danish Data Protection Agency.
All the data processed before the statistical analysis was made without knowledge of the diagnostic status of the subjects.
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