Before starting the experiments, plants were acclimatized for 1 h in aerated 0.5 mM CaCl2 solutions.
Before starting the experiments, the nematodes were kept at 25 °C.
This culture was maintained under lab condition for several generations before starting the experiments.
The animals were allowed to acclimatize in the laboratory for 5 weeks before starting the experiments.
Figure 1 depicts a smooth and a clean inner surface of an elbow before starting the experiments.
Before starting the experiments, all of the oligonucleotide samples were denatured (5 min at 95°C) to remove aggregates.
We obtained informed consent from all the participants before starting the experiments, and no form of compensation was given.
Before starting the experiments, the oligonucleotide samples were denatured (5 min at 95°C) to dissociate aggregates and were gradually cooled to room temperature.
Before starting the experiments, the cultures were allowed to grow exponentially for 30 days and the filaments were harvested by centrifugation and freeze dried.
Confluent PC-3 human prostate cancer cells were seeded in six-well plates (~1.0 × 106 cells) 24 h before starting the experiments.
Nevertheless, as interference of nanoparticles with tetrazolium-based assays is widely recognized, before starting the experiments, the credibility of the MTT assay in regards to nanozeolite was tested in a cell-free system.
