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The mixing time was about 40 45 s before starting the absorbance measurement.
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Before starting the reaction, the mixture without the ATPase was incubated at 37°C for 2 minutes and then NADH absorbance was recorded for 3 minutes and used as background to account for spontaneous ATP hydrolysis.
This question was asked before starting the events.
Before starting the production, different concepts have been studied.
Save before starting the battle.
Save the game before starting the encounter.
Make preparations before starting the hunt.
Do adequate research before starting the program.
Disconnect the spark plug before starting.
Just after starting the irradiation, the absorbance level increased to plateau at moderate levels.
Before starting, plan the whole thing out.
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