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Before staining, the medium was aspirated and the cells washed gently with PBS.
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Before staining the sections were deplasticized in dimetoxyethylacetate and rehydrated.
Before staining, the sections were deparaffinated and rehydrated.
Before staining, work the surface from a medium (150) to a fine (220) grit sandpaper.
A biofilm assay was performed essentially as previously described; bacteria were grown overnight in 2 ml of LB medium in 15 ml polystyrene tubes before staining with crystal violet to visualise the biofilm [113].
Culture medium was removed before staining, and cells were washed three times for 5 min in phosphate-buffered saline (PBS).
The culture medium was then replaced with fresh serum-free DMEM before staining with Laurdan.
All cells were washed twice and resuspended in complete RPMI-5 medium containing 5% fetal calf serum (FCS) before staining.
I then stained the wood a nice medium color and finally got some books out of boxes and arranged them, by subject, on my new shelves.
Rather, they are stains from the medium mixed with the paint -- which is flat and without texture.
(C ) Immunofluorescence of control and SETD2 RNAi depleted U2OS cells before and 8 hr after NCS treatment was performed with antibodies against RPA and cyclin A. Dapi was added to the mounting medium to stain the DNA.
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