Sentence examples for before solutions were added from inspiring English sources
The 4 mM stock solutions of the conjugates in DMSO were diluted to 100 μM with a Cremophor formulation mixture composed of PBS/EtOH/Cremophor (18 1 1 v/v/v), before solutions were added to the wells.
The stock solutions were added to the autoclaved medium directly before pouring the plates.
For each sample, 50 μL of RNAase and 450 μL of PI solution were added before incubation for 30 min at 4°C.
A 100 μl of 5 M solution of sodium chloride and subsequently a 100 μl pre-warmed cetyltrimethyl ammonium bromide (CTAB /sodium chloride solution were added before incubating in water bath at 65°C for 10 minutes.
The extract was evaporated to dryness under nitrogen stream and then subjected to derivatisation with 20 μL of N,O-bis trimethylsilyl trifluoroacetamide (BSTFA), 200 μL of isooctaN,O-bis trimethylsilyl trifluoroacetamideat 60°C for 30 min. 5 μL of hexadecane solution were added just BSTFAe injection.
300 μL blood were mixed gently with 300 μL 0.9% NaCl and incubated for 3 minutes at 37°C before 20 μL of the respective agonist solution were added.
Propidium iodide (PI, Sigma-Aldrich) and Hoechst dye solution were added 40 minutes before the end of treatments to each well at a final concentration of 1 μg/ml for each dye.
The resulting solutions were allowed to equilibrate for 30 min before 10 equiv of standardized Fe(ClO4 3 solution were added, and the solutions were gently mixed for 30 min and then allowed to stand overnight at 4 °C to precipitate any unreacted iron, which was removed by filtration with a syringe-driven 0.2 μM filter unit (Corning Corporation).
After 36 hr of drug treatment, 15 μL of tetrazolium dye solution were added, and cells were incubated for 1 hr at 37°C before adding stop solution.
The SE medium was prepared as follows: a range of SE slurries (150, 170, 190, 210 and 230 °C) were centrifuged and supernatants were transferred into sealed bottles that were subsequently autoclaved and then cooled before yeast solution was added.
To avoid gas bubbles on the hydrophobic surface, ethanol was pumped through the ellipsometry cuvette before the aqueous solution was added.
