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Therefore, it is often necessary to store surface water samples before the quantification of pharmaceutical products, pesticides and steroidal hormones.
Before seepage quantification, air injection and liquid release tests at two niche locations were conducted to characterize the fracture flow paths.
After washes, infection was allowed to proceed for 16 hours before analysis quantification of CHIKV-positive cells by flow cytometry.
Before metabolite quantification, MR signals were apodized (exponential multiplication using LB = 0.5 Hz), Fourier transformed, and phase and baseline corrected.
All the primers were validated before relative quantification.
Threshold fluorescence was obtained for each image before the quantification.
Lanes containing radioactive products were divided into one-cm fractions before radioactivity quantification.
Absolute quantification approach was used to quantify qPCR results where a standard curve of a serial dilution of virus was plotted before the quantification.
Each RNA sample was eluted with 100 μl nuclease-free water before RNA quantification with a Nanodrop apparatus (NanoDrop Lite, Thermo Scientific).
Following transfection, neurons are cultured additional 1, 3 or 5 days before imaging and quantification of total dendritic length.
However, challenges remain before renal perfusion quantification with CEUS can be used in clinical practice and further studies are required to validate its measurements.
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