Sentence examples for before sequencing by using from inspiring English sources

PCR products were then purified before sequencing by using ThermoScientific PCR purification kit (ThermoScientific Rockford, Illinois, USA) according to the manufacturer's protocols.

Before sequencing by using ABI PRISM 310 Genetic Analyzer (Applied Biosystems, California, USA) samples were purified by ExTerminator kit (Ecoli, Bratislava, Slovakia) as described by the manufacturer.

Some PCR products were cloned before sequencing by using the Topo TA Cloning Kit for Sequencing (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's directions.

PCR products were purified (QIAquick PCR Purification Kit; QIAGEN, Crawley, UK) before direct sequencing by using the PCR primers and dideoxy chain termination/cycle sequencing on an ABI 3730XL DNA sequencer (MWG Operon, Ebersberg, Germany).

Unincorporated primers were removed from samples producing amplicons before sequence determination by using ExoSAP-IT (USB Corporation, Cleveland, OH, USA).

Before tree construction, 75 groups were created from the ≈300 available picobirnavirus sequences by using FastGroup II (10 ).

Libraries were sequenced by using the Illumina platform.

Also, when I was a kid, we learned about a rainbow's color sequence by using the acronym Roy G Biv.

Before data embedding in image I, image blocks must be rearranged arbitrarily according to a random sequence RS by using a secret key SK.

All sequences were aligned before inspection by using the MUSCLE algorithm in MEGA5.2 and hand-edited for final correction (24 ).

The 16 kDa protein shown in Fig. 1A was isolated, digested with trypsin and individual peptides separated by liquid chromatography before sequencing using tandem mass spectrometry and analysis with the programme Mascot (Perkins et al., 1999) (Fig. S1).