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RNA samples were pooled in equal amounts before sequencing.
If necessary, amplicons were gel purified before sequencing.
PCR products were then purified before sequencing by using ThermoScientific PCR purification kit (ThermoScientific Rockford, Illinois, USA) according to the manufacturer's protocols.
The cDNA synthesis employing the NSR primer set was performed on all four RNA samples before sequencing on the IIllumina GA2 platform, producing single-end 80-nt reads.
Hence, the question is who is able to provide accurate information to patients before sequencing and a skilled interpretation of the results afterwards.
ETS1 was amplified by PCR before sequencing.
The resulting strand-specific cDNA was amplified before sequencing.
PCR products were purified using SPRI beads before sequencing.
The library was size-selected (120 170 bp) before sequencing.
The amplified DNA was purified by ethanol precipitation before sequencing.
Products were cloned into TOPO vectors before sequencing.
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