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The same parameters were used as before, sequence identity ≥60% and the longest sequence kept.
We also generated a plasmid with two tracer mutations, which allowed us to prepare mixtures with different, defined amounts of wild type and mutant plasmid before sequence analysis, in order to determine the sensitivity of the sequencers for picking out the occurrence of the introduced SNPs.
Though the 11-year truism has gripped the public imagination and captured the fancy of a few scientists as well, "it's misinterpretation of old data, generated before the origins of epidemics became clear, or before sequence analysis on the virus genome was possible," says Brian Murphy, chief of the respiratory virus laboratory at the National Institute of Allergy and Infectious Diseases.
(4) Denoising and chimera removal: before sequence clustering, denoising and chimera removal are required for 454 and Illumina datasets.
Kimura predicted that silent substitutions would evolve more rapidly than replacement substitutions before sequence data were available to test his neutral theory of molecular evolution.
Dr. Wu first acknowledged the Sanger sequencing as a great breakthrough, and continued to state the neglected fact: "However, the method was still based on my location specific-primer-extension principle in labeling the DNA before sequence analysis".
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Quantification and size distribution of the final library was determined as described above before sequencing.
On the "Intervention" section of A&E's Web site, intoxicated figures stumble and slump in the "before" sequences, leading up to their stints in rehab.
If necessary, amplicons were gel purified before sequencing.
RNA samples were pooled in equal amounts before sequencing.
In addition, genetic analysis revealed the presence of new pathogens, never before sequenced.
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CEO of Professional Science Editing for Scientists @ prosciediting.com