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The culture substrates were assembled before seeding of the cells.
Next, the chips were primed in RPMI-1640 for 30 minutes and subsequently in supplemented RPMI-1640 for at least 30 minutes before seeding of cells.
The GzmB/inhibitor media solution was transferred to wells that had been previously coated with FN and blocked as described above, before seeding of cells.
For measurement of angiogenic factors in the media of MDA-MB-435 cells, samples were taken before seeding of cells (base medium) and immediately before treatments (t0).
Looking more closely at the subcellular localisation of this E-cadherin and EGFR expression, freshly isolated GFP-expressing primary rat hepatocytes were allowed to adhere 24 h before seeding of the RFP-expressing prostate cancer cells.
For HUVEC cells, 5 μl of 2% type 2 gelatine (Sigma) was added to the wells and the plates were preincubated for 20 min at 37°C before seeding of cells.
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To determine the long-term effects of rhRLN2 on cell motility, MDA-MB-231 were incubated for seven days with 500 ng/ml rhRLN2 before seeding on top of the filter with culture medium containing 1% FCS and 500 ng/ml rhRLN2.
In between reading, they played in the waves for hours (until I yanked them out before seeds of melanoma took root), then caught bluefish for dinner.
Nevertheless, expansion of MC3T3-E1 cells before seeding on chitosan membranes should be performed in αMEM media for best growth behaviour.
To provide better exposure towards the extract, the UE-12 extract was spread on the NG agar before the seeding of heat-killed E. coli OP50.
All plots were supplied with 150 kg/ha of N, including 40 kg/ha of N as compound fertilizer applied before seeding, and 110 kg/ha of N as urea supplied at two-leaf stage and booting stage, respectively with equal amount.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com