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Offset potentials were compensated before seal formation.
Added to the bathing solution 2 3 min before seal formation, vanadate did not modify the initial diversity of current amplitudes, but the declining pattern persisted albeit at a significantly reduced rate (Figure 5).
The pipette potential was zeroed before seal formation, and voltages were not corrected for liquid junction potential.
The pipette potential was zeroed before seal formation and note that the amplitude of Na+ currents was not affected over the time period.
Membrane currents were recorded using the conventional whole-cell patch clamp technique using an Axopatch 200A or a Multiclamp 700A patch-clamp amplifier (Molecular Devices Co .. Liquid junction potential was nulled with the offset circuit of the amplifier before seal formation.
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