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Before scratching, the sample was cleaned thoroughly by sonication in acetone and ethanol, respectively and then rinsed with deionized water.
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One hour before scratching, the medium was replaced with medium containing 0.1% FBS to minimize the cell proliferation.
Cells were serum starved for 3 h in OptiMEM® before scratching the confluent monolayer with a P200 pipette tip.
Stop scratching the bite.
Avoid scratching the floor.
The medium was changed 16 hr before scratching.
Six dogs scratched at the sampled areas for 1 to 2 days.
To test the Fak inhibitor, cultures were pretreated 60 min before scratching.
The test basically consists in scratching a sample surface, knowing the scratching conditions and morphology.
The day before the 1995 Belmont Stakes, he scratched the heavily favored Timber Country because of a fever.
The results indicated that HF solution could selectively etch the scratched Si/Si3N4 sample.
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