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Samples were air-dried before scanning using a diode array spectrophotometer covering the wavelength range from 399 to 1697 nm.
After 3 washes (1 hours 68°C, 0.1× SSC 0.2% SDS), arrays were exposed 60 hours to phosphor-imaging plates before scanning using a FUJI BAS 5000.
After 3 washes (1 hours 68°C, 0.1× SSC 0.2% SDS), arrays were exposed 65 hours to phosphor-imaging plates before scanning using a FUJI BAS 5000.
After 3 washes (1 hour 68°C, 0.1 SSC 0.2% SDS), arrays were exposed for 65 hours to phosphor-imaging plates before scanning using a FUJI BAS 5000.
After 3 washes (1 hours 68°C, 0.1× SSC, 0.2% SDS), they were exposed to phosphor-imaging plates (24 or 48 hours) before scanning using a FUJI BAS 5000 (Fujifilm France, St . Quentin en Yvelines, France.
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Microarrays were washed for 10 min in 6X SSC, 0.005% Triton X-102, for 5 min in 0.1X SSC, 0.005% Triton X-102, and were then dried by centrifugation before scanning using an Axon 4000B microarray scanner and the GenePix Pro 6® software (Molecular Devices, Berks, UK).
Participants provided these cue phrases before the day of fMRI scanning using a procedure developed in prior research64: they first wrote a specific description of break-up experience with their ex-partner and positive experience with their friend.
Practise sessions were given before scanning using different trait terms.
Image cytometry was carried out on thin sections of FFPE tissue and areas of interest marked before scanning using Automated Cellular Imaging System.
Labelled RNA (15 μg) was fragmented and the mouse genome 430 2.0 arrays were hybridized overnight and washed as described before being scanned using a GeneChip scanner 3000 (Affymetrix).
A transmission scan was performed for attenuation correction before the emission scans (using a computed tomography scan).
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