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Microdialysis samples were collected every 30 minutes, weighed and 2 × 3 μl were taken from each sample in order to determine the activity (in duplicate) of the labelled glucose before samples were frozen at -80°C.
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The protein concentration of the dialyzed samples was measured with a BCA Protein Assay Kit Thermo Scientificc, Rockford, IL, USA) before the samples were frozen.
Once filtered, cells were killed with a 5 ml rinse of cold 10% TCA before the filtered samples were frozen in liquid nitrogen and stored at −80 °C.
Blood samples were frozen before shipping.
For logistical reasons, most of the samples were frozen before or during their shipment to the laboratory, where they were stored frozen at −21°C.
Many samples were frozen before or during their transport to the laboratory, and all were put into a freezer upon arrival in the laboratory.
However, the number of clinical cases caused by Gram negative bacteria and A. pyogenes may be underestimated because the samples were frozen before bacteriological analysis [ 34, 35].
To assess the impact of different sample treatment between ESTHER study participants (stool samples were mailed before freezing) and CRC patients (stool samples were frozen immediately), sensitivity analyses were carried out based on the results of stability testing (Haug et al, 2006).
All samples were frozen at -80°C before analysis.
Immediately after preparation, the extracted PMN samples were frozen at −80°C before lyophilisation (freeze dryer CIT-2®, Heraeus, Hanau, Germany).
Perishable samples were frozen at −80°C before shipping on dry ice to a Eurofins laboratory in Hamburg, Germany.
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